Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nucleus. 2010 Nov-Dec;1(6):460-71. doi: 10.4161/nucl.1.6.13513. Epub 2010 Sep 3.

Mapping of protein- and chromatin-interactions at the nuclear lamina.

Author information

  • 1Center for Heart Failure Research, Maastricht, The Netherlands.

Abstract

The nuclear envelope and the lamina define the nuclear periphery and are implicated in many nuclear processes including chromatin organization, transcription and DNA replication. Mutations in lamin A proteins, major components of the lamina, interfere with these functions and cause a set of phenotypically diverse diseases referred to as laminopathies. The phenotypic diversity of laminopathies is thought to be the result of alterations in specific protein- and chromatin interactions due to lamin A mutations. Systematic identification of lamin A-protein and -chromatin interactions will be critical to uncover the molecular etiology of laminopathies. Here we summarize and critically discuss recent technology to analyze lamina-protein and-chromatin interactions.

KEYWORDS:

DamID; affinity purification; chromatin immunoprecipitation; chromatin interactions; immunoprecipitation; lamin A; laminopathies; mass spectrometry; oneSTrEP; protein interactions

PMID:
21327087
[PubMed - indexed for MEDLINE]
PMCID:
PMC3027047
Free PMC Article

Images from this publication.See all images (2)Free text

Figure 1
Figure 2
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Landes Bioscience Icon for PubMed Central
    Loading ...
    Write to the Help Desk