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J R Soc Interface. 2011 Aug 7;8(61):1204-11. doi: 10.1098/rsif.2010.0747. Epub 2011 Feb 16.

Surface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulin.

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  • 1School of Engineering and Materials Science, Queen Mary, University of London, Mile End Road, London E1 4NS, UK.

Abstract

The detection sensitivity of silver nanoparticle (AgNP)-tagged goat immunoglobulin G (gIgG) microarrays was investigated by studying surface plasmon resonance (SPR) images captured in the visible wavelength range with the help of a Kretchmann-configured optical coupling set-up. The functionalization of anti-gIgG molecules on the AgNP surface was studied using transmission electron microscopy, photon correlation measurements and UV-visible absorption spectroscopy. A value of 1.3×10(7) M(-1) was obtained for the antibody-antigen binding constant by monitoring the binding events at a particular resonance wavelength. The detection limit of this SPR imaging instrument is 6.66 nM of gIgG achieved through signal enhancement by a factor of larger than 4 owing to nanoparticle tagging with the antibody.

PMID:
21325318
[PubMed - indexed for MEDLINE]
PMCID:
PMC3119885
Free PMC Article

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