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Nat Methods. 2011 Mar;8(3):242-5. doi: 10.1038/nmeth.1569. Epub 2011 Feb 6.

High-throughput single-molecule optofluidic analysis.

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  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.


We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

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