Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2011 Mar;8(3):242-5. doi: 10.1038/nmeth.1569. Epub 2011 Feb 6.

High-throughput single-molecule optofluidic analysis.

Author information

  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.

Abstract

We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

Comment in

PMID:
21297618
[PubMed - indexed for MEDLINE]
PMCID:
PMC3075913
Free PMC Article

Images from this publication.See all images (2)Free text

Figure 1
Figure 2
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Write to the Help Desk