Am J Trop Med Hyg. 2011 Feb;84(2):308-12.

Comparison of microscopy, culture, and conventional polymerase chain reaction for detection of blastocystis sp. in clinical stool samples.

Roberts T, Barratt J, Harkness J, Ellis J, Stark D.

Source

Department of Microbiology, SydPath, St. Vincent's Hospital, Sydney, Australia. troberts@stvincents.com.au

Abstract

We tested 513 stool samples from patients in Sydney, Australia for Blastocystis by using five diagnostic techniques: microscopy of a permanently stained smear using a modified iron-hematoxylin stain, two xenic culture systems (a modified Boeck and Drbohlav's medium and tryptone, yeast extract, glucose, methionine-9 medium), and two published conventional polymerase chain reaction methods specific for the small subunit ribosomal DNA. Ninety-eight (19%) samples were positive for Blastocystis in one or more of the diagnostic techniques. The PCR 2 method was the most sensitive at detecting Blastocystis with a sensitivity of 94%, and the least sensitive was microscopy of the permanent stain (48%). Subtype 3 was the most predominant subtype (present in 43% of samples assigned to this group). This study highlights the low sensitivity of microscopy when used as the sole diagnostic modality for detection of Blastocystis sp.

PMID:: 21292905; [PubMed - indexed for MEDLINE]
PMCID:: PMC3029188

Free PMC Article

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Medical

Bowel Movement - MedlinePlus Health Information

Supplemental Content

Related citations

Comparison of microscopy, two xenic culture techniques, conventional and real-time PCR for the detection of Dientamoeba fragilis in clinical stool samples. [Eur J Clin Microbiol Infect Di...]
Comparison of microscopy, two xenic culture techniques, conventional and real-time PCR for the detection of Dientamoeba fragilis in clinical stool samples.
Stark D, Barratt J, Roberts T, Marriott D, Harkness J, Ellis J. Eur J Clin Microbiol Infect Dis. 2010 Apr; 29(4):411-6. Epub 2010 Feb 14.
Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies. [J Clin Microbiol. 2011]
Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies.
Poirier P, Wawrzyniak I, Albert A, El Alaoui H, Delbac F, Livrelli V. J Clin Microbiol. 2011 Mar; 49(3):975-83. Epub 2010 Dec 22.
Molecular epidemiology of human Blastocystis isolates in France. [Parasitol Res. 2009]
Molecular epidemiology of human Blastocystis isolates in France.
Souppart L, Sanciu G, Cian A, Wawrzyniak I, Delbac F, Capron M, Dei-Cas E, Boorom K, Delhaes L, Viscogliosi E. Parasitol Res. 2009 Aug; 105(2):413-21. Epub 2009 Mar 17.
Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples. [Parasitol Res. 2011]
Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples.
Yoshikawa H, Dogruman-Ai F, Turk S, Kustimur S, Balaban N, Sultan N. Parasitol Res. 2011 Oct; 109(4):1045-50. Epub 2011 Apr 16.
Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study. [Diagn Microbiol Infect Dis. 2007]
Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study.
Stensvold CR, Arendrup MC, Jespersgaard C, Mølbak K, Nielsen HV. Diagn Microbiol Infect Dis. 2007 Nov; 59(3):303-7. Epub 2007 Oct 29.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Free in PMC
Free full text articles in PMC

Recent activity

Clear Turn Off Turn On

Comparison of microscopy, culture, and conventional polymerase chain reaction fo...
Comparison of microscopy, culture, and conventional polymerase chain reaction for detection of blastocystis sp. in clinical stool samples.
Am J Trop Med Hyg. 2011 Feb ;84(2):308-12.

PubMed
Intestinal protozoa infections among patients with ulcerative colitis: prevalenc...
Intestinal protozoa infections among patients with ulcerative colitis: prevalence and impact on clinical disease course.
Digestion. 2010 ;82(1):18-23. Epub 2010 Feb 9 .

PubMed
In vitro sensitivity of Blastocystis hominis to garlic, ginger, white cumin, and...
In vitro sensitivity of Blastocystis hominis to garlic, ginger, white cumin, and black pepper used in diet.
Parasitol Res. 2011 Aug ;109(2):379-85. Epub 2011 Mar 23 .

PubMed
Maximum Flux Transition Paths of Conformational Change.
Maximum Flux Transition Paths of Conformational Change.
J Chem Theory Comput. 2010 Aug 10;6(8):2411-2423.

PubMed
Hair analysis: another approach for the assessment of human exposure to selected...
Hair analysis: another approach for the assessment of human exposure to selected persistent organochlorine pollutants.
Chemosphere. 2002 Jan ;46(3):413-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to:

Comparison of microscopy, culture, and conventional polymerase chain reaction for detection of blastocystis sp. in clinical stool samples.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical

Supplemental Content

Related citations

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information