a, Per cent of all cytosines on each strand of the human genome assayed for each sample. b, c, The per cent of all sequencing base calls that were methylated (C, resistant to bisulphite conversion) at covered C bases in the CG (b) and CH contexts (c) (where H = A, C, or T) throughout the genome, minus the bisulphite non-conversion frequency. d, AnnoJ data browser representation of the restoration of non-CG methylation in all iPSC and ES cell lines. e, Dendrogram of the analysed cell lines based on Pearson correlation of mCG or mCH levels in 1-kb windows throughout the genome.

a, Total length of PMDs identified in each cell line and overlap of PMDs identified in the four somatic cell types. b, mRNA-Seq RPKM (reads per kilobase of exon per million reads) values for all RefSeq genes outside PMDs, and all RefSeq genes within genomic regions defined as PMDs. For ADS-iPSC and H1 the ADS PMD genomic regions were used as PMDs. P value is from two-tailed Wilcoxon test between ADS PMDs and ADS-iPSC PMDs.

a, Complete linkage hierarchical clustering of mCG density within CG-DMRs identified between all ES cell and iPSC DNA methylomes. Each CG-DMR was profiled over 20 equally sized bins. b, The CG-DMRs for each iPSC line with respect to H1 and H9 ES cells were categorized as having methylation patterns like the progenitor somatic cell line (memory) or iPSC-specific (iDMR). c, Number of iPSC hypomethylated and hypermethyated CG-DMRs aberrant in the indicated number of iPSC lines. d, Number of all CG-DMRs coincident with indicated genomic and genic features. CGI, CG island; TES, transcriptional end site; TSS, transcriptional start site.

a, Normalized mCG levels (lower y-axis) and normalized H3K27me3 ChIP-Seq read density (upper y-axis) over CG-DMRs hypermethylated in all iPSC lines and flanking genomic regions. b, Data browser representation of mRNA, DNA methylation and H3K27me3 density for a CG-DMR identified in all iPSC lines. c, Complete linkage hierarchical clustering of mCG density within the CG-DMRs hypomethylated in both FF-iPSC 19.11 and FF-iPSC 19.11-BMP4 relative to H1, H9 and H1-BMP4 cell lines. Each CG-DMR was profiled over 20 equally sized bins. d, Same as c for hypermethylated CG-DMRs. e, FF-iPSC 19.11 CG-DMR transmission through differentiation to trophoblast cells. CG-DMRs were categorized by methylation state relative to the ES cells (hyper, hypermethylated; hypo, hypomethylated), similarity to somatic progenitor methylation (memory: like progenitor; iDMR: unlike progenitor), and whether the CG-DMR was present in FF-iPSC 19.11 differentiated into trophoblast cells with BMP4 (transmitted) or not (not transmitted).

a, Chromosome ideograms and length distribution (inset) of the 22 ADS-iPSC non-CG mega-DMRs. Blue circles and lines indicate location of individual DMRs. Red ellipses indicate the location of centromeres. b, Normalized mCH levels over all non-CG mega-DMRs and flanking genomic regions. c, Lower y-axis as in b for the cell lines indicated. Upper y-axis shows normalized H3K9me3 ChIP-Seq read density throughout the non-CG mega-DMRs and flanking genomic regions. Dashed blue arrows indicate the inverse relationship between mCH and H3K9me3. d, Plot shows normalized mCG levels over the non-CG mega-DMRs and flanking genomic regions. Inset is a data browser representation of DNA methylation where vertical bar height indicates mC level at the 5′ of a non-CG mega-DMR and PMD. e, Normalized mCH levels over a non-CG mega-DMR on chromosome 22 and flanking regions. Top panel shows gene models and ADS-iPSC mCH. f, Comparison of transcript abundance between H1 and ADS-iPSC. Each dot represents a RefSeq gene within the 22 non-CG mega-DMRs. Red dots indicate genes that have a CG-DMR within 2 kb of the transcriptional start site. Blue dots indicate genes that have a CG-DMR within 2 kb of the transcriptional start site, are hypermethylated in all iPSC lines and are associated with loss of H3K27me3. Red dashed lines represent twofold difference. g, The number of genes with a given transcript abundance ratio between H1 and ADS-iPSCs for all RefSeq genes within the non-CG mega-DMRs.