J Am Chem Soc. 2011 Feb 23;133(7):2025-7. Epub 2011 Feb 2.

Measuring single small molecule binding via rupture forces of a split aptamer.

Nguyen TH, Steinbock LJ, Butt HJ, Helm M, Berger R.

Source

Max Planck Institute for Polymer Research, 55128 Mainz, Germany.

Abstract

The rupture force of a split (bipartite) aptamer that forms binding pockets for adenosine monophosphate (AMP) was measured by atomic force spectroscopy. Changes in the rupture force were observed in the presence of AMP, while this effect was absent when mutant aptamers or inosine were used. Thus, changes in the rupture force were a direct consequence of specific binding of AMP to the split aptamer. The split aptamer concept allowed the detection of nonlabeled AMP and enabled us to determine the dissociation constant on a single-molecule level.

PMID:: 21287983; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

ADENOSINE 5'-PHOSPHATE - HSDB

Supplemental Content

Related citations

Energy landscape of aptamer/protein complexes studied by single-molecule force spectroscopy. [Chem Asian J. 2007]
Energy landscape of aptamer/protein complexes studied by single-molecule force spectroscopy.
Yu J, Jiang Y, Ma X, Lin Y, Fang X. Chem Asian J. 2007 Feb 5; 2(2):284-9.
Molecular interaction studies of vascular endothelial growth factor with RNA aptamers. [Analyst. 2010]
Molecular interaction studies of vascular endothelial growth factor with RNA aptamers.
Zhang X, Yadavalli VK. Analyst. 2010 Aug; 135(8):2014-21. Epub 2010 Jun 19.
Following aptamer-thrombin binding by force measurements. [Anal Chem. 2006]
Following aptamer-thrombin binding by force measurements.
Basnar B, Elnathan R, Willner I. Anal Chem. 2006 Jun 1; 78(11):3638-42.
Characterization of enhanced monovalent and bivalent thrombin DNA aptamer binding using single molecule force spectroscopy. [Biophys J. 2011]
Characterization of enhanced monovalent and bivalent thrombin DNA aptamer binding using single molecule force spectroscopy.
Neundlinger I, Poturnayova A, Karpisova I, Rankl C, Hinterdorfer P, Snejdarkova M, Hianik T, Ebner A. Biophys J. 2011 Oct 5; 101(7):1781-7.
Review Aptamers biosensors for pharmaceutical compounds. [Comb Chem High Throughput Scre...]
Review Aptamers biosensors for pharmaceutical compounds.
Tombelli S, Mascini M. Comb Chem High Throughput Screen. 2010 Aug; 13(7):641-9.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

Measuring single small molecule binding via rupture forces of a split aptamer.
Measuring single small molecule binding via rupture forces of a split aptamer.
J Am Chem Soc. 2011 Feb 23 ;133(7):2025-7. Epub 2011 Feb 2 .

PubMed
Radical prostatectomy versus watchful waiting in early prostate cancer.
Radical prostatectomy versus watchful waiting in early prostate cancer.
N Engl J Med. 2011 May 5 ;364(18):1708-17.

PubMed
Nationwide register-based surveillance system on drugs and pregnancy in Finland ...
Nationwide register-based surveillance system on drugs and pregnancy in Finland 1996-2006.
Pharmacoepidemiol Drug Saf. 2011 Jul ;20(7):729-38. doi: 10.1002/pds.2159. Epub 2011 May 27 .

PubMed
Hydrogen Sulfide Inhibits High Glucose-induced Matrix Protein Synthesis by Activ...
Hydrogen Sulfide Inhibits High Glucose-induced Matrix Protein Synthesis by Activating AMP-activated Protein Kinase in Renal Epithelial Cells.
J Biol Chem. 2012 Feb 10 ;287(7):4451-61. Epub 2011 Dec 9 .

PubMed
Female boxing in Italy: 2002-2007 report.
Female boxing in Italy: 2002-2007 report.
Br J Sports Med. 2011 Jun ;45(7):563-70. Epub 2009 Aug 19 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: