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Methods Mol Biol. 2011;711:271-80. doi: 10.1007/978-1-61737-992-5_13.

CEST MRI reporter genes.

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  • 1Kennedy Krieger Institute, F.M. Kirby Research Center for Functional Brain Imaging, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.


In recent years, several reporter genes have been developed that can serve as a beacon for non-invasive magnetic resonance imaging (MRI). Here, we provide a brief summary of recent advances in MRI reporter gene technology, as well as detailed "hands-on" protocols for cloning, expression, and imaging of reporter genes based on chemical exchange saturation transfer (CEST).

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