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Methods Mol Biol. 2011;711:271-80. doi: 10.1007/978-1-61737-992-5_13.

CEST MRI reporter genes.

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  • 1Kennedy Krieger Institute, F.M. Kirby Research Center for Functional Brain Imaging, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. guanshu@mri.jhu.edu


In recent years, several reporter genes have been developed that can serve as a beacon for non-invasive magnetic resonance imaging (MRI). Here, we provide a brief summary of recent advances in MRI reporter gene technology, as well as detailed "hands-on" protocols for cloning, expression, and imaging of reporter genes based on chemical exchange saturation transfer (CEST).

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