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Lab Chip. 2011 Mar 7;11(5):841-4. doi: 10.1039/c0lc00498g. Epub 2011 Jan 27.

Photoactivated capture molecule immobilization in plasmonic nanoapertures in the ultraviolet.

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  • 1Department of Electrical and Computer Engineering, University of Utah, 50 South Central campus Drive, Room 3280, Salt Lake City, UT 84112, USA.

Abstract

We demonstrate a photoactivated surface coupling scheme for achieving spatial overlap between biomolecules of interest and optical near field excitation. Using aluminium nanoapertures, we obtained increased coupling efficiency of biotinylated capture probe oligos to the photoactivated surface due to ~3× nanoaperture enhancement of UV light. We further validate DNA sensor functionality via the hybridization of Cy-5 labeled target oligos, with up to 8× fluorescence enhancement obtained from a commercial microarray scanner. This generic photoimmobilization strategy is an essential step to realizing miniaturized plasmon enhanced detection arrays by virtue of localizing capture molecules to the region of plasmonic enhancement.

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