Lipopolysaccharide-stimulated activation of murine DC2.4 cells is attenuated by n-butylidenephthalide through suppression of the NF-κB pathway

Biotechnol Lett. 2011 May;33(5):903-10. doi: 10.1007/s10529-011-0528-5. Epub 2011 Jan 26.

Abstract

Modulation of dendritic cell (DC) fate and function may be one approach for the treatment of inflammatory and autoimmune diseases. n-Butylidenephthalide (BP), derived from Angelica sinensis, at 40 μg/ml significantly decreased the secretion of interleukin-6 and tumor necrosis factor-α by lipopolysaccharide (LPS)-stimulated activation of cultured murine DC2.4 cells (P<0.01). LPS-induced major histocompatibility complex class II (P<0.05), CD86 (P<0.01) and CD40 (P<0.01) expression on DC2.4 cells was also inhibited by BP. The endocytic capacity of LPS-stimulated DC2.4 cells was increased by BP (P<0.01). The antigen-presenting capacity of LPS-stimulated DC2.4 cells was decreased by BP (P<0.05). Moreover, we confirmed BP attenuates the responses of LPS-stimulated activation of DCs via suppression of NF-κB-dependent pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angelica sinensis / chemistry
  • Animals
  • B7-2 Antigen / biosynthesis
  • CD40 Antigens / biosynthesis
  • Cell Line
  • Dendritic Cells / drug effects*
  • Histocompatibility Antigens Class II / biosynthesis
  • Immunosuppressive Agents / metabolism*
  • Lipopolysaccharides / immunology*
  • Mice
  • NF-kappa B / antagonists & inhibitors*
  • Phthalic Anhydrides / isolation & purification
  • Phthalic Anhydrides / metabolism*

Substances

  • B7-2 Antigen
  • CD40 Antigens
  • Cd86 protein, mouse
  • Histocompatibility Antigens Class II
  • Immunosuppressive Agents
  • Lipopolysaccharides
  • NF-kappa B
  • Phthalic Anhydrides
  • butylidenephthalide