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PLoS One. 2011 Jan 6;6(1):e15925. doi: 10.1371/journal.pone.0015925.

PoPoolation: a toolbox for population genetic analysis of next generation sequencing data from pooled individuals.

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  • 1Institute of Population Genetics, Vetmeduni Vienna, Vienna, Austria.


Recent statistical analyses suggest that sequencing of pooled samples provides a cost effective approach to determine genome-wide population genetic parameters. Here we introduce PoPoolation, a toolbox specifically designed for the population genetic analysis of sequence data from pooled individuals. PoPoolation calculates estimates of θ(Watterson), θ(π), and Tajima's D that account for the bias introduced by pooling and sequencing errors, as well as divergence between species. Results of genome-wide analyses can be graphically displayed in a sliding window plot. PoPoolation is written in Perl and R and it builds on commonly used data formats. Its source code can be downloaded from Furthermore, we evaluate the influence of mapping algorithms, sequencing errors, and read coverage on the accuracy of population genetic parameter estimates from pooled data.

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