Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme

Guoqing Zhang; Qing Cao; Na Li; Kean Li; Feng Liu

doi:10.1016/j.talanta.2010.11.040

Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme

Talanta. 2011 Feb 15;83(5):1515-20. doi: 10.1016/j.talanta.2010.11.040. Epub 2010 Nov 26.

Authors

Guoqing Zhang¹, Qing Cao, Na Li, Kean Li, Feng Liu

Affiliation

¹ Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

PMID: 21238746
DOI: 10.1016/j.talanta.2010.11.040

Abstract

A novel affinity purification method for lysozyme (LZM) based on functionalized magnetic microspheres was developed. Tris(hydroxymethyl)aminomethane (Tris)-modified magnetic microspheres with specific affinity toward LZM were prepared using Tris as ligand and silica-coated magnetic microshperes as support. Transmission electron microscopy and magnetic property measurement results showed that the Tris-modified magnetic microspheres have a very good core-shell structure and high magnetization.The maximum binding capacity of LZM was about 108.6 mg/g magnetic microspheres. LZM purified from chicken egg white had high purity and well-maintained activity of 8140 U/mg. This magnetic-mediated LZM purification strategy has advantages of high efficiency, low cost and easy operation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Buffers
Chromatography, Affinity
Ferric Compounds / chemistry
Hydrogen-Ion Concentration
Magnetics*
Metal Nanoparticles / chemistry
Microscopy, Electron, Transmission
Microspheres
Muramidase / chemistry*
Tromethamine / chemistry*

Substances

Buffers
Ferric Compounds
Tromethamine
ferric oxide
Muramidase