Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
N Biotechnol. 2011 Jul;28(4):302-11. doi: 10.1016/j.nbt.2010.12.005. Epub 2011 Jan 11.

Surrogate antigens as targets for proteome-wide binder selection.

Author information

  • 1Department of Immunotechnology, Lund University, SE-221 84 Lund, Sweden.

Abstract

In the past decade, many initiatives were taken for the development of antibodies for proteome-wide studies, as well as characterisation and validation of clinically relevant disease biomarkers. Phage display offers many advantages compared to antibody generation by immunisation because it is an unlimited resource of affinity reagents without batch-to-batch variation and is also amendable for high throughput in contrast to conventional hybridoma technology. One of the major bottlenecks to proteome-wide binder selection is the limited supply of suitable target antigens representative of the human proteome. Here, we provide proof of principle of using easily accessible, cancer-associated protein epitope signature tags (PrESTs), routinely generated within the Human Protein Atlas project, as surrogate antigens for full-length proteins in phage selections for the retrieval of target-specific binders. These binders were subsequently tested in western blot, immunohistochemistry and protein microarray application to demonstrate their functionality.

Copyright © 2011 Elsevier B.V. All rights reserved.

PMID:
21232647
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk