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Curr Protoc Mol Biol. 2011 Jan;Chapter 13:Unit13.10B. doi: 10.1002/0471142727.mb1310bs93.

Conditional depletion of nuclear proteins by the Anchor Away system.

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  • 1Harvard Medical School, Boston, Massachusetts, USA.

Abstract

Nuclear proteins play key roles in the regulation of many important cellular processes. In Saccharomyces cerevisiae, many genes encoding nuclear proteins are essential. This unit describes a method termed Anchor Away that can be used to conditionally and rapidly deplete nuclear proteins of interest. It involves conditional export of the protein of interest out of the nucleus and its subsequent sequestration in the cytoplasm. This method can be used to simultaneously deplete multiple proteins from the nucleus.

PMID:
21225637
[PubMed - indexed for MEDLINE]
PMCID:
PMC3076635
Free PMC Article

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