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Biochem Biophys Res Commun. 2011 Feb 4;405(1):74-8. doi: 10.1016/j.bbrc.2010.12.130. Epub 2011 Jan 5.

Evidence for specific interaction between the RhoGAP domain from the yeast Rgd1 protein and phosphoinositides.

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  • 1Université de Bordeaux, Chimie et Biologie des Membranes et des Nanoobjets, CNRS UMR 5248, Pessac, France.

Abstract

The Rho GTPase activating protein Rgd1 increases the GTPase activity of Rho3p and Rho4p, which are involved in bud growth and cytokinesis, respectively, in the budding yeast Saccharomyces cerevisiae. Rgd1p is a member of the F-BAR family conserved in eukaryotes; indeed, in addition to the C-terminal RhoGAP domain Rgd1p possesses an F-BAR domain at its N-terminus. Phosphoinositides discriminate between the GTPase activities of Rho3p and Rho4p through Rgd1p and specifically stimulate the RhoGAP activity of Rgd1p on Rho4p. Determining specific interactions and resolving the structure of Rgd1p should provide insight into the functioning of this family of protein. We report the preparation of highly pure and functional RhoGAP domain of Rgd1 RhoGAP domain using a high yield expression procedure. By gel filtration and circular dichroïsm we provide the first evidences for a specific interaction between a RhoGAP domain (the RhoGAP domain of Rgd1p) and phosphoinositides.

Copyright © 2011 Elsevier Inc. All rights reserved.

PMID:
21215255
[PubMed - indexed for MEDLINE]
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