Industrial poultry workers may be at elevated risk of avian influenza infection due to intense occupational contact with live poultry. Serum samples from poultry workers and community members in the Delmarva Peninsula, one of the densest regions of poultry production in the United States, were analyzed for antibodies to strains of five avian influenza subtypes using microneutralization assays. No evidence of infection was found, suggesting inefficient transmission to humans or the absence of virus in these premises. Continued serological surveillance of workers in industrial food animal facilities is necessary to prevent the transmission of influenza A viruses.