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Oxidative Stress at the Single Cell Level.


In: Michael AC, Borland LM, editors.


Electrochemical Methods for Neuroscience. Boca Raton (FL): CRC Press; 2007. Chapter 13.


The studies described here demonstrate that the electrochemical measurements at microelectrodes are methods of high interest for studying biological processes implicating superoxide and/or nitric oxide and their derivatives. The advantages of the electrochemical analysis over other biophysical methods include the possible direct detection, the fast response-time and selectivity of the sensors, and, above all, a single cell analysis. Clearly, when the microelectrode surface can be positioned at close distance to the cell (or the biological source), i.e., in an artificial synapse configuration, the infinitely low amplitude of the biological information can even so be detected and studied. Future developments of the electrochemical methodologies for studies of oxidative stress processes should focus on (1) the improvement of microelectrodes selectivity for each ROS and RNS of interest; (2) multiple analysis on several cells or of several species done in parallel by several electrodes eventually integrated into microsystems; (3) intracellular measurements owing to nanometer size electrodes; (4) detection of a single molecule activity such as the one of an enzymatic system generating O2 •− or NO , possibly located into the cell cytosol or the cell membrane.

Copyright © 2007, Taylor & Francis Group, LLC

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