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PLoS One. 2010 Dec 23;5(12):e15801. doi: 10.1371/journal.pone.0015801.

Targeted amino-terminal acetylation of recombinant proteins in E. coli.

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  • 1School of Biosciences, University of Kent, Canterbury, United Kingdom.

Abstract

One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function. By co-expressing the fission yeast NatB complex with the target protein in E.coli, we report a simple and widely applicable method for the expression and purification of functional N-terminally acetylated eukaryotic proteins.

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