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Genes Dev. 2011 Jan 15;25(2):119-24. doi: 10.1101/gad.1998711. Epub 2010 Dec 31.

miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1.

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  • 1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.

Abstract

Using RNA-seq technology, we found that the majority of microRNAs (miRNAs) present in CFU-E erythroid progenitors are down-regulated during terminal erythroid differentiation. Of the developmentally down-regulated miRNAs, ectopic overexpression of miR-191 blocks erythroid enucleation but has minor effects on proliferation and differentiation. We identified two erythroid-enriched and developmentally up-regulated genes, Riok3 and Mxi1, as direct targets of miR-191. Knockdown of either Riok3 or Mxi1 blocks enucleation, and either physiological overexpression of miR-191 or knockdown of Riok3 or Mxi1 blocks chromatin condensation. Thus, down-regulation of miR-191 is essential for erythroid chromatin condensation and enucleation by allowing up-regulation of Riok3 and Mxi1.

PMID:
21196494
[PubMed - indexed for MEDLINE]
PMCID:
PMC3022257
Free PMC Article

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