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PLoS One. 2010 Dec 20;5(12):e15651. doi: 10.1371/journal.pone.0015651.

Tiling histone H3 lysine 4 and 27 methylation in zebrafish using high-density microarrays.

Author information

  • 1Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, and Norwegian Center for Stem Cell Research, Oslo, Norway.

Abstract

BACKGROUND:

Uncovering epigenetic states by chromatin immunoprecipitation and microarray hybridization (ChIP-chip) has significantly contributed to the understanding of gene regulation at the genome-scale level. Many studies have been carried out in mice and humans; however limited high-resolution information exists to date for non-mammalian vertebrate species.

PRINCIPAL FINDINGS:

We report a 2.1-million feature high-resolution Nimblegen tiling microarray for ChIP-chip interrogations of epigenetic states in zebrafish (Danio rerio). The array covers 251 megabases of the genome at 92 base-pair resolution. It includes ∼15 kb of upstream regulatory sequences encompassing all RefSeq promoters, and over 5 kb in the 5' end of coding regions. We identify with high reproducibility, in a fibroblast cell line, promoters enriched in H3K4me3, H3K27me3 or co-enriched in both modifications. ChIP-qPCR and sequential ChIP experiments validate the ChIP-chip data and support the co-enrichment of trimethylated H3K4 and H3K27 on a subset of genes. H3K4me3- and/or H3K27me3-enriched genes are associated with distinct transcriptional status and are linked to distinct functional categories.

CONCLUSIONS:

We have designed and validated for the scientific community a comprehensive high-resolution tiling microarray for investigations of epigenetic states in zebrafish, a widely used developmental and disease model organism.

PMID:
21187971
[PubMed - indexed for MEDLINE]
PMCID:
PMC3004928
Free PMC Article

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