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PLoS One. 2010 Dec 20;5(12):e15271. doi: 10.1371/journal.pone.0015271.

The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe.

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  • 1Institute for Genome Sciences and Policy, Duke University Medical Center, Durham, North Carolina, United States of America.

Abstract

A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe.

PMID:
21187966
[PubMed - indexed for MEDLINE]
PMCID:
PMC3004915
Free PMC Article
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