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Methods. 2011 May;54(1):4-15. doi: 10.1016/j.ymeth.2010.12.029. Epub 2010 Dec 25.

Using Lamm-Equation modeling of sedimentation velocity data to determine the kinetic and thermodynamic properties of macromolecular interactions.

Author information

  • Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, TX 75390-8816, USA. chad.brautigam@utsouthwestern.edu

Abstract

The interaction of macromolecules with themselves and with other macromolecules is fundamental to the functioning of living systems. Recent advances in the analysis of sedimentation velocity (SV) data obtained by analytical ultracentrifugation allow the experimenter to determine important features of such interactions, including the equilibrium association constant and information about the kinetic off-rate of the interaction. The determination of these parameters is made possible by the ability of modern software to fit numerical solutions of the Lamm Equation with kinetic considerations directly to SV data. Herein, the SV analytical advances implemented in the software package SEDPHAT are summarized. Detailed analyses of SV data using these strategies are presented. Finally, a few highlights of recent literature reports that feature this type of SV data analysis are surveyed.

Copyright © 2010 Elsevier Inc. All rights reserved.

PMID:
21187153
[PubMed - indexed for MEDLINE]
PMCID:
PMC3147155
Free PMC Article

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