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Methods. 2011 Apr;53(4):366-71. doi: 10.1016/j.ymeth.2010.12.022. Epub 2010 Dec 23.

Genetic screens using the piggyBac transposon.

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  • 1Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK.

Abstract

Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments.

Copyright © 2010 Elsevier Inc. All rights reserved.

PMID:
21185377
[PubMed - indexed for MEDLINE]
PMCID:
PMC3650578
Free PMC Article

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