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Clin Cancer Res. 2011 Mar 15;17(6):1362-72. doi: 10.1158/1078-0432.CCR-10-2213. Epub 2010 Dec 21.

Chondroitinase ABC I-mediated enhancement of oncolytic virus spread and antitumor efficacy.

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  • 1Dardinger Laboratory for Neuro-oncology and Neurosciences, Department of Neurological Surgery, Center for Biostatistics, The Ohio State University Medical Center, Columbus, Ohio, USA.

Abstract

PURPOSE:

The inhibitory role of secreted chondroitin sulfate proteoglycans on oncolytic viral (OV) therapy was examined. Chondroitinase ABC (Chase-ABC) is a bacterial enzyme that can remove chondroitin sulfate glycosaminoglycans from proteoglycans without any deleterious effects in vivo. We examined the effect of Chase-ABC on OV spread and efficacy.

EXPERIMENTAL DESIGN:

Three-dimensional glioma spheroids placed on cultured brain slices were utilized to evaluate OV spread. Replication-conditional OV-expressing Chase-ABC (OV-Chase) was engineered using HSQuik technology and tested for spread and efficacy in glioma spheroids. Subcutaneous and intracranial glioma xenografts were utilized to compare antitumor efficacy of OV-Chase, rHsvQ (control), and PBS. Titration of viral particles was performed from OV-treated subcutaneous tumors. Glioma invasion was assessed in collagen-embedded glioma spheroids in vitro and in intracranial tumors. All statistical tests were two sided.

RESULTS:

Treatment with Chase-ABC in cultured glioma cells significantly enhanced OV spread in glioma spheroids grown on brain slices (P < 0.0001). Inoculation of subcutaneous glioma xenografts with Chase-expressing OV significantly increased viral titer (>10 times, P = 0.0008), inhibited tumor growth, and significantly increased overall animal survival (P < 0.006) compared with treatment with parental rHsvQ virus. Single OV-Chase administration in intracranial xenografts also resulted in longer median survival of animals than rHsvQ treatment (32 vs. 21 days, P < 0.018). Glioma cell migration and invasion were not increased by OV-Chase treatment.

CONCLUSIONS:

We conclude that degradation of glioma extracellular matrix with OV-expressing bacterial Chase-ABC enhanced OV spread and antitumor efficacy.

©2010 AACR.

PMID:
21177410
[PubMed - indexed for MEDLINE]
PMCID:
PMC3140790
Free PMC Article

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