Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Phys Chem B. 2011 Jan 20;115(2):366-75. doi: 10.1021/jp1083357. Epub 2010 Dec 21.

Role of cationic group structure in membrane binding and disruption by amphiphilic copolymers.

Author information

  • 1Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, Michigan 48109, USA.

Abstract

Cationic, amphiphilic polymers are currently being used as antimicrobial agents that disrupt biomembranes, although their mechanisms remain poorly understood. Herein, membrane association and disruption by amphiphilic polymers bearing primary, tertiary, or quaternary ammonium salt groups reveal the role of cationic group structure in the polymer-membrane interaction. The dissociation constants of polymers to liposomes of POPC were obtained by a fluorometric assay, exploiting the environmental sensitivity of dansyl moieties in the polymer end groups. Dye leakage from liposomes and solid-state NMR provided further insights into the polymer-induced membrane disruption. Interestingly, the polymers with primary amine groups induced reorganization of the bilayer structure to align lipid headgroups perpendicular to the membrane. The results showed that polymers bearing primary amines exceed the tertiary and quaternary ammonium counterparts in membrane binding and disrupting abilities. This is likely due to enhanced complexation of primary amines to the phosphate groups in the lipids, through a combination of hydrogen bonding and electrostatic interactions.

PMID:
21171655
[PubMed - indexed for MEDLINE]
PMCID:
PMC3021096
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Write to the Help Desk