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J Neurochem. 2011 Feb;116(4):606-15. doi: 10.1111/j.1471-4159.2010.07144.x. Epub 2011 Jan 19.

Activation of TRPV4 channels reduces migration of immortalized neuroendocrine cells.

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  • 1Dipartimento di Scienze Chimiche, Alimentari, Farmaceutiche e Farmacologiche, Universit√† degli Studi del Piemonte Orientale A. Avogadro, Novara, Italy.

Abstract

Calcium is a universal signal, and its capacity to encode intracellular messages via spatial, temporal and amplitude characteristics allows it to participate in most cellular events. In a specific context, calcium plays a pivotal role in migration, although its role has not been elucidated fully. By using immortalized gonadotropin-releasing hormone-secreting neurons (GN11), we have now investigated the role of TRPV4, a member of the vanilloid family of Ca(2+) channels, in neuronal migration. Our results show that TRPV4 channels are present and functional in GN11 cells and their localization is polarized and enriched in lamellipodial structures. TRPV4 activation leads to a retraction of the lamellipodia and to a decrease in migratory behaviour; moreover cells migrate slower and in a more random manner. We therefore provide evidence for a new regulation of gonadotropin-releasing hormone neurons and a new role for calcium at the leading edge of migratory cells.

© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry.

PMID:
21166676
[PubMed - indexed for MEDLINE]
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