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Opt Lett. 2010 Dec 15;35(24):4102-4. doi: 10.1364/OL.35.004102.

Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy.

Author information

  • 1École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. etienne.shaffer@epfl.ch

Abstract

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.

PMID:
21165103
[PubMed - indexed for MEDLINE]
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