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J Am Chem Soc. 2011 Jan 26;133(3):420-3. doi: 10.1021/ja109630v. Epub 2010 Dec 16.

Light regulation of protein dimerization and kinase activity in living cells using photocaged rapamycin and engineered FKBP.

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  • 1Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.

Abstract

We developed a new system for light-induced protein dimerization in living cells using a photocaged analogue of rapamycin together with an engineered rapamycin binding domain. Using focal adhesion kinase as a target, we demonstrated successful light-mediated regulation of protein interaction and localization in living cells. Modification of this approach enabled light-triggered activation of a protein kinase and initiation of kinase-induced phenotypic changes in vivo.

PMID:
21162531
[PubMed - indexed for MEDLINE]
PMCID:
PMC3133816
Free PMC Article

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