[Establishment of a HEK293 cell line stably expressing human HCN2 gene]

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006 May;22(2):254-6.
[Article in Chinese]

Abstract

Aim: To create a model for studying ionic channels by means of the expressing human HCN2 and G418-resistant HEK293 cell lines established.

Methods: pcDNA3-hHCN2 was transfected with Lipofectin2000 into HEK293 cell line. The transfected cells would be survived in the further culture medium containing G418 antibiotic as the hHCN2 gene could express a G418 resistant products. Whole-cell patch clamp investigated that hHCN2 gene was transfected into HEK293 cells.

Results: The G418 resistant (600 ug/ml) HEK293 cell line was established successfully and whole-cell patch clamp recorded ionic currents of transfected hHCN2.

Conclusion: The G418 resistant HEK293 cell line was successfully established with transfection of plasmid pcDNA3-hHCN2 by Lipofectin, which might be useful for studying the relationship between the structure and function of cloned ionic channels.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression
  • Genetic Vectors*
  • HEK293 Cells*
  • Humans
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
  • Ion Channels / genetics*
  • Patch-Clamp Techniques
  • Plasmids
  • Potassium Channels
  • Transfection

Substances

  • HCN2 protein, human
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
  • Ion Channels
  • Potassium Channels