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World J Hepatol. 2010 Mar 27;2(3):127-35. doi: 10.4254/wjh.v2.i3.127.

Proteomic analysis for developing new biomarkers of hepatocellular carcinoma.

Author information

  • 1Maria Pleguezuelo, Ruben Ciria, Liver Research Unit and Academic Department of Surgery, Reina Sofia University Hospital, Avda Menendez Pidal s/n, Cordoba 14004, Spain.

Abstract

AIM:

To identify new markers of hepatocellular carcinoma (HCC) using a proteomic analysis.

METHODS:

Patients with liver cirrhosis of the three most frequent etiologies: hepatitis C virus, hepatitis B virus and alcoholic liver disease, were included in the study. The samples were analysed by 2D-electrophoresis in order to determine the differential protein expression. The proteins were separated according to the charge in immobilized pH 3-10 gradient strips and then by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Proteins of interest were excised, digested with trypsin and the resulting peptides were separated and identified.

RESULTS:

Three differentially expressed apolipoproteins (Apo) were identified based on the protein profile using proteomic techniques: Apo-A1, Apo-A4 and Apo-E. Apo-A4 levels were significantly lower in HCC than in non-HCC patients regardless of etiology (P < 0.01). Multivariate logistic regression showed that Apo-A4 and Apo-A1 were the only independent factors related to HCC diagnosis (P < 0.05). The receiver operating characteristic (ROC) curve including both Apo-A4 and Apo-A1 showed an area under the ROC of 0.944 (P < 0.001), a sensitivity of 0.89 and a specificity of 0.81 for diagnosis of HCC.

CONCLUSION:

Apo-A4 and Apo-A1 may be used clinically as biomarkers of HCC with a high sensibility and specificity. These findings may provide additional insights into the mechanism of HCC development and progression.

KEYWORDS:

2D polyacrylamide gel electrophoresis; Apolipoproteins; Liver cancer; Mass spectrometry; Serum biomarkers

PMID:
21160983
[PubMed]
PMCID:
PMC2998961
Free PMC Article

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