A. We plot, as a function of number of supporting reads, the fraction of junctions 1) matching GT-AG, the splice site consensus sequences (black), 2) matching a control pair of dinucleotides (grey), 3) annotated in EST databases (light blue), or 4) annotated in gene databases (dark blue). B. We split all junctions into those that are annotated in gene model databases and those that are not. Plotted is the cumulative number of junctions of each type by expression level. Unannotated junctions are expressed at much lower levels than annotated junctions. C and D. Alternative splice junctions near known protein-coding junctions show a periodic pattern. At each alternatively-spliced protein-coding 3′ or 5′ splice site, we counted the positions of AG (or GT, respectively) dinucleotides used as alternative splice sites, then averaged this across splice sites (see Methods). The red points denote positions that are a multiple of three base pairs from the major splice form, and the black points those that are not. The blue box below each panel shows the position of the exon.

In the top panel, we plot the average expression level at each base in a region surrounding HERPUD1. In blue are bases annotated as exonic, and in black are those annotated as not exonic. In the middle panel, we plot the positions of all splice junctions in the region identified in our data. In black are splice junctions that are present in gene databases; in red are those that are not. The number of sequencing reads supporting each junction is written to the right of each junction, and junctions are ordered from top to bottom of the plot according to their coverage. In the bottom panel, we show the gene models in the region from Ensembl. The blue boxes show the positions of exons, and the black lines the positions of introns.

In each panel, we plot the mean phyloP score [31] at each base surrounding the splice site. In the top panels are annotated splice sites, and in the bottom panels are unannotated splice sites. In blue are bases exonic of the splice site, and in black are those intronic of the splice site, as diagrammed below each panel.

We divided all introns that are bounded by highly conserved splice sites into 100 bins based on length. We then calculated, in each bin, the mean fraction of sequencing reads from either splice site to an unconserved splice site. Plotted is this mean against the of the mean intron length (in base pairs) of introns in the bin. In red is a spline fit to these points.

A. Plotted is the enrichment of all possible hexamers exonic of either 5′ or 3′ noise splice sites. In light blue are hexamers identified as exonic splicing enhancers by Fairbrother et al. [35], and in dark blue are hexamers that are good matches to the consensus U1 snSNP binding site (we include all hexamers matching five contiguous bases of “AGGTAAG”). B and C. Hexamers from A. mark borders of constitutively spliced exons. Each point is the fraction of hexamers starting at that position relative to a constitutively spliced exon (in these cells) which match the hexamers identified as significantly enriched exonic or intronic of the “noise” 5′ or 3′ splice sites.