Format

Send to:

Choose Destination
See comment in PubMed Commons below
Immunity. 2010 Dec 14;33(6):905-16. doi: 10.1016/j.immuni.2010.11.023. Epub 2010 Dec 9.

Continuous expression of the transcription factor e2-2 maintains the cell fate of mature plasmacytoid dendritic cells.

Author information

  • 1Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032, USA.

Abstract

The interferon-producing plasmacytoid dendritic cells (pDCs) share common progenitors with antigen-presenting classical dendritic cells (cDCs), yet they possess distinct morphology and molecular features resembling those of lymphocytes. It is unclear whether the unique cell fate of pDCs is actively maintained in the steady state. We report that the deletion of transcription factor E2-2 from mature peripheral pDCs caused their spontaneous differentiation into cells with cDC properties. This included the loss of pDC markers, increase in MHC class II expression and T cell priming capacity, acquisition of dendritic morphology, and induction of cDC signature genes. Genome-wide chromatin immunoprecipitation revealed direct binding of E2-2 to key pDC-specific and lymphoid genes, as well as to certain genes enriched in cDCs. Thus, E2-2 actively maintains the cell fate of mature pDCs and opposes the "default" cDC fate, in part through direct regulation of lineage-specific gene expression programs.

PMID:
21145760
[PubMed - indexed for MEDLINE]
PMCID:
PMC3010277
Free PMC Article

Publication Types, MeSH Terms, Substances, Grant Support

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk