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Genome Biol. 2010;11(12):R119. doi: 10.1186/gb-2010-11-12-r119. Epub 2010 Dec 8.

Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.

Author information

  • 1Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA. acadey@uw.edu

Abstract

We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.

PMID:
21143862
[PubMed - indexed for MEDLINE]
PMCID:
PMC3046479
Free PMC Article

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