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Nat Biotechnol. 2011 Jan;29(1):79-83. doi: 10.1038/nbt.1720. Epub 2010 Dec 5.

Toolkit for evaluating genes required for proliferation and survival using tetracycline-regulated RNAi.

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  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.

Abstract

Short hairpin RNAs (shRNAs) are versatile tools for analyzing loss-of-function phenotypes in vitro and in vivo. However, their use for studying genes involved in proliferation and survival, which are potential therapeutic targets in cancer and other diseases, is confounded by the strong selective advantage of cells in which shRNA expression is inefficient. We therefore developed a toolkit that combines Tet-regulated miR30-shRNA technology, robust transactivator expression and two fluorescent reporters to track and isolate cells with potent target knockdown. We demonstrated that this system improves the study of essential genes and was sufficiently robust to eradicate aggressive cancer in mice by suppressing a single gene. Further, we applied this system for in vivo negative-selection screening with pooled shRNAs and propose a streamlined, inexpensive workflow that will facilitate the use of RNA interference (RNAi) for the identification and evaluation of essential therapeutic targets.

PMID:
21131983
[PubMed - indexed for MEDLINE]
PMCID:
PMC3394154
Free PMC Article

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