Format

Send to:

Choose Destination
See comment in PubMed Commons below
BMC Mol Biol. 2010 Dec 2;11:91. doi: 10.1186/1471-2199-11-91.

Essential role for ALCAM gene silencing in megakaryocytic differentiation of K562 cells.

Author information

  • 1Department of Pediatrics, Aflac Cancer Center, Emory University School of Medicine, Atlanta, GA 30322, USA.

Abstract

BACKGROUND:

Activated leukocyte cell adhesion molecule (ALCAM/CD166) is expressed by hematopoietic stem cells. However, its role in hematopoietic differentiation has not previously been defined.

RESULTS:

In this study, we show that ALCAM expression is silenced in erythromegakaryocytic progenitor cell lines. In agreement with this finding, the ALCAM promoter is occupied by GATA-1 in vivo, and a cognate motif at -850 inhibited promoter activity in K562 and MEG-01 cells. Gain-of-function studies showed that ALCAM clusters K562 cells in a process that requires PKC. Induction of megakaryocytic differentiation in K562 clones expressing ALCAM activated PKC-δ and triggered apoptosis.

CONCLUSIONS:

There is a lineage-specific silencing of ALCAM in bi-potential erythromegakaryocytic progenitor cell lines. Marked apoptosis of ALCAM-expressing K562 clones treated with PMA suggests that aberrant ALCAM expression in erythromegakaryocytic progenitors may contribute to megakaryocytopenia.

PMID:
21126364
[PubMed - indexed for MEDLINE]
PMCID:
PMC3003670
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Write to the Help Desk