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Opt Lett. 2010 Dec 1;35(23):4066-8. doi: 10.1364/OL.35.004066.

Optofluidic system for three-dimensional sensing and identification of micro-organisms with digital holographic microscopy.

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  • 1Department of Electrical and Computer Engineering, University of Connecticut, Storrs, Connecticut 06269-2157, USA.

Abstract

Optofluidic devices offer flexibility for a variety of tasks involving biological specimen. We propose a system for three-dimensional (3D) sensing and identification of biological micro-organisms. This system consists of a microfluidic device along with a digital holographic microscope and relevant statistical recognition algorithms. The microfluidic channel is used to house the micro-organisms, while the holographic microscope and a CCD camera record their digital holograms. The holograms can be computationally reconstructed in 3D using a variety of algorithms, such as the Fresnel transform. Statistical recognition algorithms are used to analyze and identify the micro-organisms from the reconstructed wavefront. Experimental results are presented. Because of computational reconstruction of wavefronts in holographic imaging, this technique offers unique advantages that allow one to image micro-organisms within a deep channel while removing the inherent microfluidic-induced aberration through interferometery.

PMID:
21124614
[PubMed - indexed for MEDLINE]
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