Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
DNA Repair (Amst). 2011 Jan 2;10(1):102-10. doi: 10.1016/j.dnarep.2010.10.004. Epub 2010 Nov 5.

Chromosome integrity at a double-strand break requires exonuclease 1 and MRX.

Author information

  • 1National Institute of Environmental Health Sciences, NIH, Laboratory of Molecular Genetics, Research Triangle Park, NC 27709, USA.

Abstract

The continuity of duplex DNA is generally considered a prerequisite for chromosome continuity. However, as previously shown in yeast as well as human cells, the introduction of a double-strand break (DSB) does not generate a chromosome break (CRB) in yeast or human cells. The transition from DSB to CRB was found to be under limited control by the tethering function of the RAD50/MRE11/XRS2 (MRX) complex. Using a system for differential fluorescent marking of both sides of an endonuclease-induced DSB in single cells, we found that nearly all DSBs are converted to CRBs in cells lacking both exonuclease 1 (EXO1) activity and MRX complex. Thus, it appears that some feature of exonuclease processing or resection at a DSB is critical for maintaining broken chromosome ends in close proximity. In addition, we discovered a thermal sensitive (cold) component to CRB formation in an MRX mutant that has implications for chromosome end mobility and/or end-processing.

Published by Elsevier B.V.

PMID:
21115410
[PubMed - indexed for MEDLINE]
PMCID:
PMC3031249
Free PMC Article

Images from this publication.See all images (6)Free text

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk