Format

Send to:

Choose Destination
See comment in PubMed Commons below
Cell Stem Cell. 2010 Dec 3;7(6):671-81. doi: 10.1016/j.stem.2010.11.012.

MicroRNA profiling reveals two distinct p53-related human pluripotent stem cell states.

Author information

  • 1Neuroscience Research Institute, University of California at Santa Barbara, 93106, USA.

Abstract

Reprogramming methodologies have provided multiple routes for achieving pluripotency. However, pluripotency is generally considered to be an almost singular state, with subtle differences described between induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). We profiled miRNA expression levels across 49 human cell lines, including ESCs, iPSCs, differentiated cells, and cancer cell lines. We found that the resulting miRNA profiles divided the iPSCs and hESCs examined into two distinct categories irrespective of the cell line origin. The miRNAs that defined these two pluripotency categories also distinguished cancer cells from differentiated cells. Transcriptome analysis suggested that several gene sets related to p53 distinguished these categories, and overexpression of the p53-targeting miRNAs miR-92 and miR-141 in iPSCs was sufficient to change their classification status. Thus, our results suggest a subdivision of pluripotent stem cell states that is independent of their origin but related to p53 network status.

Copyright © 2010 Elsevier Inc. All rights reserved.

Comment in

PMID:
21112562
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk