Send to:

Choose Destination
See comment in PubMed Commons below
BMB Rep. 2010 Nov;43(11):720-5. doi: 10.5483/BMBRep.2010.43.11.720.

Identification of SUMOylated proteins in neuroblastoma cells after treatment with hydrogen peroxide or ascorbate.

Author information

  • 1School of Dentistry, University of Birmingham, Birmingham, B4 6NN, UK.


The small ubiquitin-like modifier (SUMO) proteins have been implicated in the pathology of a number of diseases, including neurodegenerative diseases. The conjugation machinery for SUMOylation consists of a number of proteins which are redox sensitive. Here, under oxidative stress (100 μM hydrogen peroxide), antioxidant (100 μM ascorbate) or control conditions 169 proteins were identified by electrospray ionisation fourier transform ion cyclotron resonance mass spectrometry. The majority of these proteins (70%) were found to contain SUMOylation consensus sequences. From the remaining proteins a small number (12%) were found to contain possible SUMO interacting motifs. The proteins identified included DNA and RNA binding proteins, structural proteins and proteasomal proteins. Several of the proteins identified under oxidative stress conditions had previously been identified as SUMOylated proteins, thus validating the method presented.

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Korean Society for Biochemistry and Molecular Biology
    Loading ...
    Write to the Help Desk