An integrated, low noise patch-clamp amplifier for biological nanopore applications

Annu Int Conf IEEE Eng Med Biol Soc. 2010:2010:2718-21. doi: 10.1109/IEMBS.2010.5626570.

Abstract

We present an integrated, low noise patch-clamp amplifier for biological nanopore applications. Our amplifier consists of an integrator-differentiator architecture coupled with a novel opamp design in the CMOS 0.35 µm process. The post-layout full-chip simulation shows the input referred noise of the amplifier is 0.49 pA RMS over a 5 kHz bandwidth using a verified electrical model for the biological nanopore system. In our biological nanopore experiments studying protein-DNA interactions, we encounter capacitive transients with a nominal settling time of 5 ms. Our amplifier design reduces the settling time to 0.2 ms, without requiring any compensation circuitry.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Amplifiers, Electronic*
  • Biology / methods
  • Biomedical Engineering / methods
  • Computer Simulation
  • DNA / chemistry
  • DNA, Single-Stranded / genetics
  • Electrophysiology / methods
  • Equipment Design
  • Escherichia coli Proteins / chemistry
  • Hemolysin Proteins / chemistry
  • Kinetics
  • Nanopores*
  • Nanotechnology / methods*
  • Patch-Clamp Techniques / methods*
  • Software

Substances

  • DNA, Single-Stranded
  • Escherichia coli Proteins
  • Hemolysin Proteins
  • Hlya protein, E coli
  • DNA