Abstract
Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cell Differentiation
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Cell Division
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Cloning, Molecular
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DNA-Binding Proteins / genetics*
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Epidermal Growth Factor / pharmacology
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Gene Expression Regulation / drug effects
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Immunologic Techniques
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Metalloproteins / genetics*
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Mice
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Molecular Sequence Data
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Nerve Growth Factors / pharmacology
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Nuclear Proteins / physiology*
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Phosphoproteins / genetics
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Protein Processing, Post-Translational
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Tetradecanoylphorbol Acetate / pharmacology
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Transcription Factors / genetics*
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Zinc / physiology
Substances
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DNA-Binding Proteins
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Metalloproteins
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Nerve Growth Factors
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Nuclear Proteins
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Phosphoproteins
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Transcription Factors
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Epidermal Growth Factor
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Zinc
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Tetradecanoylphorbol Acetate