Adipogenic differentiation of adipose-derived stem cells

Methods Mol Biol. 2011:702:193-200. doi: 10.1007/978-1-61737-960-4_14.

Abstract

The primary physiological function of adipose-derived stem cells (ASCs) is to differentiate into adipose tissue. It is now possible to isolate, expand, and cryopreserve ASC from adipose depots of many animal species. These ASC can be induced to undergo adipogenic differentiation in vitro by exposure to a cocktail of chemical agents or inductive growth factors. The current chapter describes methods to induce adipogenesis and to quantify this differentiation process in vitro.

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / metabolism
  • Adipogenesis* / genetics
  • Adipose Tissue / cytology*
  • Azo Compounds / metabolism
  • Cell Culture Techniques / methods*
  • Humans
  • Organ Specificity / genetics
  • Staining and Labeling
  • Stem Cells / cytology*
  • Tissue Fixation
  • Up-Regulation / genetics

Substances

  • Azo Compounds
  • oil red O