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Chem Commun (Camb). 2011 Jan 21;47(3):973-5. doi: 10.1039/c0cc03864d. Epub 2010 Nov 16.

Ribonuclease S redux.

Author information

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706-1544, USA.

Abstract

The S-peptide and S-protein components of bovine pancreatic ribonuclease form a noncovalent complex with restored ribonucleolytic activity. Although this archetypal protein-fragment complementation system has been the object of historic work in protein chemistry, intrinsic limitations compromise its utility. Modern methods are shown to overcome those limitations and enable new applications.

PMID:
21079871
[PubMed - indexed for MEDLINE]
PMCID:
PMC3077719
Free PMC Article

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