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FEBS Lett. 2010 Dec 1;584(23):4745-50. doi: 10.1016/j.febslet.2010.11.006. Epub 2010 Nov 10.

Feedback regulation of Ras2 guanine nucleotide exchange factor (Ras2-GEF) activity of Cdc25p by Cdc25p phosphorylation in the yeast Saccharomyces cerevisiae.

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  • 1Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Nankai District, Tianjin, PR China.


Ras-GEF Cdc25p has been found to be hyperphosphorylated upon glucose addition. This work provides evidence indicating that PKA activity positively regulates the degree of Cdc25p phosphorylation, and that the intracellular association of Cdc25p and Ras2p is independent of PKA activity. In vitro experiments revealed that the Ras2-GEF activity of Cdc25p is inhibited by Cdc25p phosphorylation. These data suggest a negative feedback mechanism by which intracellular cAMP synthesis is inhibited by PKA through Cdc25p phosphorylation.

Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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