Display Settings:

Format

Send to:

Choose Destination
J Biol Chem. 2011 Jan 21;286(3):2297-307. doi: 10.1074/jbc.M110.185694. Epub 2010 Nov 11.

Evidence that prestin has at least two voltage-dependent steps.

Author information

  • 1Department of Communication Sciences and Disorders, The Hugh Knowles Center, Northwestern University, Evanston, Illinois 60208, USA. k-homma@northwestern.edu

Abstract

Prestin is a voltage-dependent membrane-spanning motor protein that confers electromotility on mammalian cochlear outer hair cells, which is essential for normal hearing of mammals. Voltage-induced charge movement in the prestin molecule is converted into mechanical work; however, little is known about the molecular mechanism of this process. For understanding the electromechanical coupling mechanism of prestin, we simultaneously measured voltage-dependent charge movement and electromotility under conditions in which the magnitudes of both charge movement and electromotility are gradually manipulated by the prestin inhibitor, salicylate. We show that the observed relationships of the charge movement and the physical displacement (q-d relations) are well represented by a three-state Boltzmann model but not by a two-state model or its previously proposed variant. Here, we suggest a molecular mechanism of prestin with at least two voltage-dependent conformational transition steps having distinct electromechanical coupling efficiencies.

PMID:
21071769
[PubMed - indexed for MEDLINE]
PMCID:
PMC3023524
Free PMC Article

Images from this publication.See all images (5)Free text

FIGURE 1.
FIGURE 2.
FIGURE 3.
FIGURE 4.
FIGURE 5.
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk