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Nat Genet. 2010 Dec;42(12):1113-7. doi: 10.1038/ng.710. Epub 2010 Nov 7.

Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells.

Author information

  • 1Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Manton Center for Orphan Disease Research, Children's Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts, USA.

Erratum in

  • Nat Genet. 2010 Dec;42(12): 3 p following 1117.

Abstract

The conversion of lineage-committed cells to induced pluripotent stem cells (iPSCs) by reprogramming is accompanied by a global remodeling of the epigenome, resulting in altered patterns of gene expression. Here we characterize the transcriptional reorganization of large intergenic non-coding RNAs (lincRNAs) that occurs upon derivation of human iPSCs and identify numerous lincRNAs whose expression is linked to pluripotency. Among these, we defined ten lincRNAs whose expression was elevated in iPSCs compared with embryonic stem cells, suggesting that their activation may promote the emergence of iPSCs. Supporting this, our results indicate that these lincRNAs are direct targets of key pluripotency transcription factors. Using loss-of-function and gain-of-function approaches, we found that one such lincRNA (lincRNA-RoR) modulates reprogramming, thus providing a first demonstration for critical functions of lincRNAs in the derivation of pluripotent stem cells.

Comment in

PMID:
21057500
[PubMed - indexed for MEDLINE]
PMCID:
PMC3040650
Free PMC Article

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