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Methods Mol Biol. 2011;681:25-33. doi: 10.1007/978-1-60761-913-0_2.

Gel-filtration chromatography.

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  • 1School of Biotechnology and National Centre for Sensor Research, Dublin City University, Dublin, Ireland. ciaran.fagan@dcu.ie


Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted. The selection of suitable operating conditions and applications of the method are also discussed.

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