(A) Three-dimensional confocal laser scanning micrograph of biofilm collected from the ABM2 location following fluorescence in situ hybridization using Leptospirillum group II (red)- and III (green)-specific oligonucleotide probes. The scale bar is equivalent to 2 µm. (B) Hierarchical clustering (Pearson uncentered) of correlated proteins and metabolite features (r value, ≥0.9285; statistical P value, ≤0.0001). Distinct organismal clusters exist for Leptospirillum group II (identified proteins are indicated by red bars on the right) and Leptospirillum group III (identified proteins are indicated by green bars on the right). Sample color coding is defined in Fig. S1 in the supplemental material.

(A) Correlation network (r value, ≥ 0.9285; statistical P value, ≤0.0001) for all of the organism-specific metabolite features as defined by the clusters in Fig. 1B. (B) Correlation network of proteins and metabolite features (r ≥ 0.9285; statistical P value ≤0.0001). The two main clusters (i and j) of correlated proteins and metabolite features identified using the graph clustering algorithm FAG-EC (10) are highlighted in yellow. (C) Correlation network (r value, ≥ 0.9285; statistical P value, ≤ 0.0001) based on protein expression data. The two main clusters of correlated proteins are highlighted in yellow. Key to the color coding of nodes in networks: dark red circles, C₁₈ reverse-phase metabolite features (primarily nonpolar metabolites) that fall within the Leptospirillum group II-specific cluster (Fig. 1B); pink circles, diamond hydride (DH) normal-phase metabolite features (primarily polar metabolites) that fall within the Leptospirillum group II-specific cluster; dark green circles, C₁₈ metabolite features that fall within the Leptospirillum group III-specific cluster; light green circles, DH metabolite features that fall within the Leptospirillum group III-specific cluster; red triangles, proteins encoded by Leptospirillum group II; green triangles, proteins encoded by Leptospirillum group III; turquoise triangles, proteins encoded by a bacterium belonging to the phylum Firmicutes; olive triangles, proteins encoded by a bacterium belonging to the phylum Actinobacteria; light blue triangles, proteins encoded by A-plasma; purple triangles, proteins encoded by G-plasma. In all of the networks, the edges are shaded according to the significance of the correlations as highlighted in panel A (black, low; white, high).