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Nat Methods. 2010 Nov;7(11):893-5. doi: 10.1038/nmeth.1521. Epub 2010 Oct 17.

Dual RMCE for efficient re-engineering of mouse mutant alleles.

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  • 1Developmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.

Abstract

We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.

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PMID:
20953177
[PubMed - indexed for MEDLINE]
PMCID:
PMC3576631
Free PMC Article

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