Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 2010 Nov 12;402(2):291-6. doi: 10.1016/j.bbrc.2010.10.017. Epub 2010 Oct 16.

Dependency of γ-secretase complex activity on the structural integrity of the bilayer.

Author information

  • 1Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA. hzhou2@lbl.gov


γ-secretase is a membrane protein complex associated with the production of Aβ peptides that are pathogenic in Alzheimer's disease. We have characterized the activity of γ-secretase complexes under a variety of detergent solubilization and reconstitution conditions, and the structural state of proteoliposomes by electron microscopy. We found that γ-secretase activity is highly dependent on the physical state or integrity of the membrane bilayer--partial solubilization may increase activity while complete solubilization will abolish it. The activity of well-solubilized γ-secretase can be restored to near native levels when properly reconstituted into a lipid bilayer environment.

Copyright © 2010 Elsevier Inc. All rights reserved.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk