Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 2010 Nov 12;402(2):291-6. doi: 10.1016/j.bbrc.2010.10.017. Epub 2010 Oct 16.

Dependency of γ-secretase complex activity on the structural integrity of the bilayer.

Author information

  • 1Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA. hzhou2@lbl.gov

Abstract

γ-secretase is a membrane protein complex associated with the production of Aβ peptides that are pathogenic in Alzheimer's disease. We have characterized the activity of γ-secretase complexes under a variety of detergent solubilization and reconstitution conditions, and the structural state of proteoliposomes by electron microscopy. We found that γ-secretase activity is highly dependent on the physical state or integrity of the membrane bilayer--partial solubilization may increase activity while complete solubilization will abolish it. The activity of well-solubilized γ-secretase can be restored to near native levels when properly reconstituted into a lipid bilayer environment.

Copyright © 2010 Elsevier Inc. All rights reserved.

PMID:
20937251
[PubMed - indexed for MEDLINE]
PMCID:
PMC2981652
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk